Optical coherence tomography for ultrahigh-resolution 3D imaging of cell development and real-time guiding for photodynamic therapy
【著者】
Tianshi Wang, Jinggao Zhen, Bo Wang, and Ping Xue
Tsinghua Univ. (China)

【概要】
Optical coherence tomography is a new emerging technique for cross-sectional imaging with high spatial resolution of micrometer scale. It enables in vivo and non-invasive imaging with no need to contact the sample and is widely used in biological and clinic application. In this paper optical coherence tomography is demonstrated for both biological and clinic applications. For biological application, a white-light interference microscope is developed for ultrahigh-resolution full-field optical coherence tomography (full-field OCT) to implement 3D imaging of biological tissue. Spatial resolution of 0.9µm×1.1µm (transverse×axial) is achieved A system sensitivity of 85 dB is obtained at an acquisition time of 5s per image. The development of a mouse embryo is studied layer by layer with our ultrahigh-resolution full-filed OCT. For clinic application, a handheld optical coherence tomography system is designed for real-time and in situ imaging of the port wine stains (PWS) patient and supplying surgery guidance for photodynamic therapy (PDT) treatment. The light source with center wavelength of 1310nm, -3 dB wavelength range of 90 nm and optical power of 9mw is utilized. Lateral resolution of 8 µm and axial resolution of 7µm at a rate of 2 frames per second and with 102dB sensitivity are achieved in biological tissue. It is shown that OCT images distinguish very well the normal and PWS tissues in clinic and are good to serve as a valuable diagnosis tool for PDT treatment.
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これ、ズバリやってた研究室があったと思います。誰でも思いつくんですね。やっぱりイメージング技術は撮ったもん勝ですなぁ。。。
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Ultra-high Resolution Measurement by Optical Coherence Tomography of Dynamic Tear Film Changes on Contact Lenses.
【著者】
Chen Q , Wang J , Tao A , Shen M , Jiao S , Lu F

【概要】
Purpose: To determine the dynamic pre- (PLTF) and post-lens (PoLTF) tear film thicknesses using optical coherence tomography (OCT). Methods: Ultra-high resolution OCT was used to image the tear film of 22 subjects before and after contact lens wear. A soft lens with one drop of artificial tears on its concave surface was inserted onto one randomly selected eye. OCT images were taken before insertion, immediately afterwards, and every 2 minutes for 10 minutes. For the contralateral eye, the lens inserted was not pre-wetted on the concave surface. OCT images were taken before insertion, immediately afterwards, and at 3 minutes. Then another drop was instilled and images taken immediately afterwards, and every 2 minutes for 10 minutes. Images were processed by custom software to yield tear film thickness. Results: The thickness of pre-corneal tear film (PCTF) was 1.9 +/- 0.9 mum. The PoLTF was visualized clearly in all cases immediately after lens insertion with one drop on its concave surface. Through the first 6 minutes after insertion, the PoLTF was greater than the PCTF. The PLTF (n = 12) and PoLTF (n = 9) were visualized immediately after lens insertion. After three minutes, the PLTF in most subjects and PoLTF in all subjects were invisible. The thickness of the PLTF increased after the instillation of artificial tears, while the PoLTF did not at any checkpoint for 10 minutes. Conclusions: Ultra-high resolution OCT is a promising tool for measuring the tear film. The PoLTF did not increase after instillation of artificial tears.
PMID: 19933178 [PubMed - as supplied by publisher]
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【ニュース】
Michelson Diagnostics presents advantages of OCT laser imaging for cancer diagnostics at RSM innovation summit
http://www.mtbeurope.info/news/2009/912012.htm
OCTのガン診断への応用。後で見る用

【プレスリリース】
Axsun Technologies, A Wholly-Owned Subsidiary of Volcano Corporation, Announces Breakthrough Integrated Light Source Architecture for Optical Coherence Tomography (OCT) Imaging
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